J. Lotze, U. Reinhardt, O. Seitz, K. Mörl, A.G. Beck-Sickinger
ACS Chem Biol.
2018, DOI: 10.1021/acschembio.7b00999
Time-Resolved Tracking of separately Internalized Neuropeptide Y(2) Receptors by Two-Color Pulse-Chase.
Link to the article


D. Schumacher, J. Helma, A.F.L. Schneider, H. Leonhardt, C.P.R. Hackenberger
Angewandte Chem.
2017, DOI: 10.1002/ange.201708459
Angewandte Chem. Int. Ed. 2017, DOI: 10.1002/anie.201708459
Chemical functionalization strategies and intracellular applications of nanobodies.
Link to the article

A. Scior, K. Arnsburg, M. Iburg, K. Juenemann, M.L. Pigazzini, B. Mlody, D. Puchkov, A. Ast, A. Buntru, J. Priller, E.E. Wanker, A. Prigione, J. Kirstein
The EMBO Jornal
2017, in press
Complete suppression of Htt fibrilization and disaggregation of Htt fibrils by a chaperone complex.

P. Rathi, S. Maurer, D. Summerer
Philos. Trans. r. Soc. Lond. B Biol. Sci.
2017, in press
Selective Recognition of N4-Methylcytosine in DNA by Engineered Transcription-Activator-Like Effectors

M.J. Schmidt, D. Summerer
Meth. Mol. Biol.
2017, in press
Directed evolution of orthogonal pyrrolysyl-t-RNA synthetases in Escherichia coli for the genetic encoding of noncanonical amino acids.

S. Middel, C.H. Panse, S. Nawratil, U. Diederichsen
Chem. Bio. Chem.
2017, 18(23), 2328-2332
Native Chemical Ligation Directed by Photocleavable Peptide Nucleic Acid (PNA) Templates
Link to the article

A.F.L. Schneider, C.P.R. Hackenberger
Curr. Opin. in Biotechnology
2017, 48, 61-68
Flourescent labeling in living cells.
Link to the article

J. Kirstein, K. Arnsburg, A. Scior, A. Szlachcic, D.L. Guilbride, R. I. Morimoto, B. Bukau, N. B. Nillegoda
Aging Cell
2017, 16(6), 1414-1424
In vivo properties of the disaggregase function of J-proteins and Hsc70 in Caenorhabditis elegans stress and aging
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P. Werther, J.S. Möhler, R. Wombacher
Chem. Eur. J.
2017, 72, 18216-18224
A Bifunctional Fluorogenic Rhodamine Probe for Proximity-Induced Bioorthogonal Chemistry.
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K. Gröger, G. Gavins, O. Seitz
Angew. Chem. Int. Ed. 2017, 56, 14217-14221
Strand Displacement in Coiled-Coil Structures - Controlled Induction and Reversal of Proximity
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A. Blees, D. Januliene, T. Hofmann, N. Koller, C. Schmidt, S. Trowitzsch, A. Moeller, R. Tampé
2017, 551, 525-528
Structure of the human MHC-I peptide-loading complex
Link to the article

M. Cigler, T.G. Müller, D. Horn-Ghetko, M.-K. von Wrisberg, M. Fottner, R.S. Goody, A. Itzen, M.P.  Müller, K. Lang
Angew. Chem. Int. Ed.
2017, DOI: 10.1002/anie.201706927
Proximity-Triggered Covalent Stabilization of Low-Affinity Protein Complexes in vitro and in vivo.
Link to the article

P. Rathi, A. Witte, D. Summerer
Sci. Rep.
2017, 7(1), 15067
Engineering DNA Backbone Interactions Results in TALE Scaffolds with Enhanced 5-Methylcytosine Selectivity.
Link to the article

L. Schmohl, J. Bierlmeier, N. von Kügelgen, L. Kurz, P. Reis, F. Barthels, P. Mach, M. Schutkowski, C. Freund, D. Schwarzer
Bioorg Med. Chem. 2017, 25, 5002-5007
Identification of sortase substrates by specificity profiling
Link to the article

A. Yang, I. Hacheney, Y.W. Wu
Bioorg & Med Chemistry
2017, 25(18), 4971-4976
Semisynthesis of qutophagy protein LC3 conjugates.
Link to the article

L. Schmohl, J. Bierlmeier, F. Gerth, C. Freund, D. Schwarzer
J Pept Sci. 2017, 23, 631-635
Engineering sortase A by screening a second-generation library using phage display
Link to the article

J.E.G.A. Dold, J. Pfotzer, A.-K. Späte, V. Wittmann
2017, 18(13), 1242-1250
Dienophile-Modified Mannosamine Derivatives for Metabolic Labeling of Sialic Acids: A Comparative Study
Link to the article

H.D. Herce, D. Schumacher, A.F.L. Schneider, A.K. Ludwig, F.A. Mann, M. Filies, M.-A. Kasper, S. Reinke, E. Krause, H. Leonhardt, M.C. Cardoso, C.P.R. Hackenberger
Nature Chemistry
2017, 9, 762-771
Cell-permeable nanobodies for targeted immunolabelling and antigen manipulation in living cells.
Link to the article

Link to supplementary movie

E. Kozma, G. Estrada Girona, G. Paci, E.A. Lemke, P. Kele
Chem Commun (Camb) 2017, 53(50), 6696-6699
Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy
Link to the article

S. Pantoom, A. Yang, Y.W. Wu
2017, 13(8), 1467-1469
Lift and cut: Anti-host autophagy mechanism of Legionella pneumophila.
Link to the article

A. Zemella, S. Grossmann, R. Sachse, A. Sonnabend, M. Schaefer, S. Kubick
Scientific Reports
2017, 7:3740, DOI: 10.1038/s41598-017-03955-8
Qualifying a eukaryotic cell-free system for fluorescence based GPCR analyses
Link to the article

V. Sakin, J. Hanne, J. Dunder, M. Anders-Össwein, V. Laketa, I. Nikic, H.G. Kräusslich, E.A. Lemke, B. Müller
Cell Chem Biol. 2017, 24(5), 635-645
A Versatile Tool for Live-Cell Imaging and Super-Resolution Nanoscopy Studies of HIV-1 Env Distribution and Mobility
Link to the article

O.Demeter, A. Kormos, C. Koehler, G. Mezo, K. Németh, E. Kozma, L.B. Takács, E.A. Lemke, P. Kele
Bioconjug Chem. 2017, 28(5), 1552-1559
Bisazide Cyanine Dyes as Fluorogenic Probes for Bis-Cyclooctynylated Peptide Tags and as Fluorogenic Cross-Linkers of Cyclooctynylated Proteins.
Link to the article

C.G.F. Graf, C. Schulz, M. Schmälzlein, C. Heinlein, M. Mönnich, L. Perkams, M. Püttner, I. Boos, M. Hessefort, J.N. Lombana Sanchez, M. Weyand,  C. Unverzagt
Angew. Chem. Int. Ed. 2017, 56(19), 5252-5257
Synthetic Glycoforms reveal Carbohydrate-Dependent Bioactivity of Human Saposin D
Link to the article

S. Flade, J. Jasper, M. Gieß, M. Juhasz, A. Dankers, G. Kubik, O. Koch, E. Weinhold, D. Summerer
ACS Chem. Biol.
2017, 12(7), 1719-1725
The N6-Position of Adenine Is a Blind Spot for TAL-Effectors That Enables Effective Binding of Methylated and Fluorophore-Labeled DNA.
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X. Chen, M. Venkatachalapathy, D. Kamps, S. Weigel, R. Kumar, M. Orlich, R. Garrecht, M. Hirtz, C.M. Niemeyer, Y.W. Wu, L. Dehmelt
Angew. Chem. Int. Ed.
2017, 56(21), 5916-5920
"Molecular Activity painting": Switch-like, Light-Controlled Perturbations inside Living Cells (Hot paper, inside cover story)
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A. Pech, J. Achenbach, M. Jahnz, S. Schülzchen, F. Jarosch, F. Bordusa, S. Klussmann
Nucleic Acids Res. 2017, 45(7), 3997-4005
A thermostable d-polymerase for mirror-image PCR
Link to the article

A.Yang, S. Pantoom, Y.W. Wu
2017, pii:e23905, DOI: 10.7554/eLife.23905
Elucidation of the anti-autophagy mechanism of the Legionella effector RavZ using semisynthetic LC3 proteins.
Link to the article

D. Schumacher, O. Lemke, J. Helma, L. Gerszonowicz, V. Waller, T. Stoschek, P.M. Durkin, N. Budisa, H. Leonhardt, B.G. Keler, C.P.R. Hackenberger
Chem. Sci.
2017, 8, 3471-3478
Broad substrate tolerance of tubulin tyrosine ligase enables one-step site-specific enzymatic protein labeling
Link to the article

A. Stengl, D. Hörl, H. Leonhardt, J. Helma
SLAS Discovery
2017, 22(3), 309-315
A Simple and Sensitive High-Content Assay for the Characterization of Antiproliferative Therapeutic Antibodies
Link to the article

A.-L. Bachmann, H.D. Mootz
J. Pep. Sci
2017, in press, DOI: 10.1002/psc.2996
N-terminal chemical protein labeling using the naturally split GOS-TerL intein.
Link to the article

T. Anton, S. Bultmann
2017, 8(3), 279-286
Site-specific recruitment of epigenetic factors with a modular CRISPR/Cas System
Link to the article

M.C. Lechler, E.D. Crawford, N. Groh, K. Widmaier, R. Jung, J. Kirstein, J.C. Ttrinidad. A.L. Burlingame. D.C. David
Cell Rep.
2017, 18(2), 454-467
Reduced Insulin/IGF-1 Signaling Restores the Dynamic Properties of Key Stress Granule Proteins during Aging.
Link to the article

R. Röder, J. Helma, T. Preiß, J.O. Rädler, H. Leonhardt, E. Wagner
Pharm. Res.
2017, 34, 161-174
Intracellular Delivery of Nanobodies for Imaging of Target Proteins in Live Cells
Link to the article

M. Braner, R. Wieneke, R. Tampé
2017, 53, 545-548
Nanomolar affinity protein trans-splicing monitored in real-time by fluorophore-quencher pairs.
Link to the article

A. Wieczorek, P. Werther, J. Euchner, R. Wombacher
2017, 8, 1506-1510
Green- to far-red-emitting fluorogenic tetrazine probes - synthetic access and no-wash protein imaging inside living cells.
Link to the article

S. Hauke, A. von Appen, T. Quidwar, J. Ries, R. Wombacher
2017, 8(1), 559-566
Specific protein labeling with caged fluorophores for dual-color imaging and super-resolution microscopy in living cells.
Link to the article

S. Palei, H.D. Mootz
Methods Mol. Biol.
2017, 1495, 77-92
Preparation of semisynthetic peptide macrocycles using split inteins.
Link to the article

S. Mayer, K. Lang
2017, 49, 830
Tetrazines in Inverse-Electron-Demand Diels-Alder Cycloadditions and Their Use in Biology
Link to the article


V. Siegmund. B. Piater, B. Zakeri, T. Eichhorn, F. Fischer, C. Deutsch, S. Becker, L. Toleikis, B. Hock, U. Betz, H. Kolmar
Sci. rep.
2016, 6, 39291
Spontaneous Isopeptide Bond Formation as a Powerful Tool for Engineering Site-Specific Antibody-Drug Conjugates
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I. Nikic, G.E. Girona, J.H. Kang, G. Paci, S. Mikhaleva, C. Koehler, N. V. Shymanska, C.V. Santos, D. Spitz, E.A. Lemke
Angew. Chem. Int. Ed.
2016, 55(52), 16172-16176
Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy
Link to the article

S. Voss, D.M. Krüger, O. Koch. Y.W. Wu
Proc. Natl. Acad. Sci USA
2016, 113(50), 14348-14343
Spatiotemporal imaging of small GTPases activity in live cells.
Link to the article

S. Maurer, M. Giess, O. Koch, D. Summerer
ACS Chem. Biol.
2016, 11, 3294-3299.
Interrogating key positions of size-reduced TALE-repeats reveals a programmable sensor of 5-carboxlcytosine
Link to the article

Y.J. Lee, M.J. Schmidt, J.M. Tharp, A. Weber, J. Gao, M.L. Waters, D. Summerer, W.R. Liu
Chem. Commun.
2016, 52(85), 12606-12609.
Genetically encoded fluorophenylalanines enable insights into the recognition of lysine trimethylation by an epigenetic reader
Link to the article

J. Bertran-Vicente, M. Penkert, O. Nieto-Garcia, J.-M. Jeckelmann, P. Schmieder, E. Krause, C.P.R. Hackenberger
Nature Comm.
2016, 7, Article number: 12703
Chemoselective synthesis and analysis of naturally occurring phosphorylated cysteine peptides.
Link to the article

L. Thoring, D.A. Wüstenhagen, M. Borowiak, M. Stech, A. Sonnabend, S. Kubick
2016, 11(9), e0163670
Cell-Free Systems Based on CHO Cell Lysates: Optimization Strategies, Synthesis of "Difficult-to-Express" Proteins and Future Perspectives.
Link to the article

R.B. Quast, B. Ballion, M. Stech, A. Sonnabend, B.R. Varga, D.A. Wüstenhagen, P. Kele, S.M. Schiller & S. Kubick
Sci. Rep.
2016, 6, 34048
Cell-Free synthesis of functional human epidermal growth factor receptor: Investigation of ligand-independent dimerization in Sf21 microsomal membranes using non-canonical amino acids.
Link to the article

A. Guesdon, F. Bazile, R.M. Buey, R. Mohan, S. Monier, R.R. Garcia, M. Angevin, C. Heichette, R. Wieneke, R. Tampé, L. Duchesne, A. Akhmanova, M.O. Steinmetz, D. Chrétien
Nature Cell Biology
2016, 8, 1102-1108
EB1 interacts with outwardly curved and straight regions of the microtubule lattice.
Link to the article

M. Mönnich, S. Eller, T. Karagiannis, L. Perkams, T. Luber, D. Ott, M. Niemietz, J. Hoffman, J. Walcher, L. Berger, M. Pischl, M. Weishaupt, C. Wirkner, R.G. Lichtenstein, C. Unverzagt
Angew. Chem.
2016, 128, 10643-10648
Angew. Chem. Int. Ed. 2016, 55, 10487-10492
Highly Efficient Synthesis of Multiantennary Bisected N-glycans Based on Imidates
Link to the article

P. Rathi, S. Maurer, G. Kubik, D. Summerer
J. Am. Chem. Soc.
2016, 138, 9910-9918
Isolation of human genomic DNA sequences with expanded nucleobase selectivity
Link to the article

L. Zhao, C. Ehrt, O. Koch, Y.W. Wu
Angew. Chem. Int. Ed.
2016, 55(28), 8129-8133.
One-Pot N2C/C2C/N2N Ligation To Trap Weak Protein-Protein Interactions.
Link to the article

A.-K. Späte, J.E.G.A. Dold, E. Batroff, V.F. Schart, D.E. Wieland, O.R. Baudendistel, V. Wittmann
2016, 17, 1274-1383
Exploring the Potential of Norbornene-Modified Mannosamine Derivatives for Metabolic Glycoengineering.
Link to the article

G. Kubik, D. Summerer
2016, 17, 975-980
TALEored epigenetics: a DNA-binding scaffold for programmable epigenome editing and analysis
Link to the article

B. Simon, A.-S. Huart, K. Temmerman, J. Vahokoski, H.D.T. Mertens, D. Komadina, J.-E. Hoffmann, H. Yumerefendi, D. Svergun, P. Kursuli, C. Schultz, D. Hart, A. McCarthy, M. Wilmanns
2016, 24, 851-861.
Death-associated protein kinase activity is regulated by coupled calcium/calmodulin binding to two distinct sites.
Link to the article

R.B. Quast, A. Sonnabend, M. Stech. D.A. Wüstenhagen, S. Kubick
Sci. Rep.
2016, 6, 30399
High-yield cell-free synthesis of human EGFR by IRES-mediated protein translation in a continuous exchange cell-free reaction format.
link to the article

S. Schwagerus, O. Reimann, C. Despres, C. Smet-Nocca, C.P.R. Hackenberger
J. Pept. Sci.
2016,22(5), 327-333
Semi-synthesis of a tag-free O-GlcNAcylated tau protein by sequential chemoselective ligation
Link to the article

H.-Y. Chiu, W. Deng, H. Engelke, J. Helma, H. Leonhardt, T. Bein
Sci. Rep.
2016, 6, 25019
Intracellular chromobody delivery by mesoporous silica nanoparticles for antigen targeting and visualization in real time.
link to the article

M. Best, I. Porth, S. Hauke, F. Braun, D.P. Herten, R. Wombacher
Org. Biomol. Chem
2016, 14(24), 5606-5611
Protein-specific localization of a rhodamine-based calcium-sensor in living cells.
Link to the article

C. Koehler, P. Sauter, M. Wawryszyn, G. Estrada Girona, K. Gupta, J.J.M. Landry, M.H. Fritz, K. Radic, J.-E. Hoffmann, Z. Angel Chen, J. Zou, P. Siong Tan, B. Galik, S. Junttila, P. Stolt-Bergner, G. Pruneri, A. Gyenesei, C. Schultz, M. Bosse Biskup, H. Besir, V. Benes, J. Rappsilber, M. Jechlinger, J.O. Korbel, I Berger, S. Braese, E.A. Lemke
Nat. Methods
2016, 13, 997-1000
Genetic code expansion for multiprotein complex engineering
Link to the article

D. Schumacher, C.P.R. Hackenberger, H. Leonhardt, J. Helma
J. Clin. Immunol.
2016, 36, 100-107 (Review)
Current Status: Site-Specific Antobody Drug Conjugates.
Link to the article

B. Valldorf, H. Fittler, L. Deweid, A. Ebenig, S. Dickgiesser, C. Sellmann, J. Becker, S. Zielonka, M. Empting, O. Avrutina, H. Kolmar
Angew. Chem. Int. Ed.
2016, DOI: 10.1002/anie.201511894
An Apoptosis-Inducing Peptidic Heptad That Efficiently Clusters Death Receptor 5.
Link to the article

S. Palei, H. Mootz
2016, 17(5), 378-382
Cyclic Peptides Made by Linking Synthetic and Genetically Encoded Fragments
Link to the article

X. Chen Y.W. Wu
Org. Biomol. Chem. 2016, 14(24), 5417-5439.
Selective chemical labeling of proteins.
Link to the article

F. Doll, A. Buntz, A.-K. Späte, V.F. Schart, A. Timper, W. Schrimpf, C.R. Hauck, A. Zumbusch, V. Wittmann
Angew. Chem. Int. Ed.
2016, 55, 2262-2266
Visualization of Protein-Specific Glycosylation inside Living Cells.
Link to the article

W. Fischle, D. Schwarzer
ACS Chemical Biology
2016, 11(3), 689-705 (Review)
Probing Chromatin-modifying enzymes with Chemical Tools.
Link to the article

A. Kollmannsperger, A. Sharei, A. Raulf, M. Heilemann, R. Langer, K.F. Jensen, R. Wieneke, R. Tampé
Nature Commun.
2016, 7, Article number 10372
Live-cell protein labelling with nanometre precision by cell squeezing
Link to the article

C. Meyer, S. Liebscher, F. Bordusa
Bioconjugate Chem
2016, 27(1), 47-53
Selective Coupling of Click Anchors to Proteins via Trypsiligase
Link to the article

A. Stützer, S. Liokatis, A. Kiesel, D. Schwarzer, R. Sprangers, J. Söding, P. Selenko, W. Fischle
Molecular Cell
2016, 61, 247-259
Modulations of DNA Contacts by Linker Histones and Post-translational Modifications Determine the Mobility and Modifiability of Nucleosomal H3 Tails.
Link to the article

A. Dose, J. Sindlinger, J. Bierlmeier, A. Bakirbas, K. Schulze-Osthoff, S. Einsele-Scholz, M. Hartl, F. Essmann, I. Finkemeier, D. Schwarzer
Angew. Chem. Int. Ed.
2016, 55, 1192-1195
Interrogating Substrate Selectivity and Composition of Endogenous Histone Deacetylase Complexes with Chemical Probes.
Link to the article

E. Hoffmann, K. Streichert, N. Nischan, C. Seitz, T. Brunner, S. Schwagerus, C.P.R. Hackenberger, M. Rubini
Molecular BioSystems
2016, DOI: 10.1039/C5MB00857C
Stabilization of bacterially expressed erytropoietin by single site-specific introduction of short branched PEG chains at naturally occurring glycosylaton sites.

M. Braner, A. Kollmannsperger, R. Wieneke, R. Tampé
2016, 7, 2646-2652
'Traceless' tracing of proteins - high-affinity transsplicing directed by a minimal interaction pair.
Link to the article


J.A. Wagner, D. Mercadante, I. Nikic, E.A. Lemke, F. Gräter
Chem. Eur. J.
2015 in press
Orthogonality of Strain-Promoted Click Reactions

J. Seidel, C. Klockenbusch, D. Schwarzer
2015, Ahead of print
Investigating deformylase and deacylase activity of mammalian and bacterial sirtuins.

M. Pfreundschuh,D. Alsteens, R. Wieneke, C. Zhang, S. Soughlin, R. Tampé, B. Kobilka, D. Müller
Nature Commun
2015, 6, 8857
Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM.
Link to the article

A.-L. Bachmann, H. Mootz
J. Biol. Chem.
2015, 290(48), 28792-28804
An Unprecedented Combination of Serine and Cysteine Nucleophiles in a Split Intein with an Atypical Split Site
Link to the article

V. Siegmund, S. Schmelz, S. dickgiesser, J. Beck, A. Ebenig, H. Fittler, H. Frauendorf, B. Piater, U.A.K. Betz, O. Avrutina, A. Scrima, H.-L. Fuchsbauer, H. Kolmar
Angew. Chem. Int. Ed.
2015, 54, 13420-13424
Locked by Design: A Conformationally Constrained Transglutaminase Tag Enables Efficient Site-Specific Conjugation
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S. Voss, L. Klewer, Y.W. Wu
Curr. Opin. Chem. Biol.
2015, 28, 194-201
Chemically induced dimerization: reversible and spatiotemporal control of protein functionin cells.
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W. Fischle, H. Mootz, D. Schwarzer
Curr. Opin. Chem. Biol.
2015, 28, 131-140 (Review)
Synthetic histone code.
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X. Chen, F. Li, Y.W. Wu
Chem. Comm.
2015, 92, 16537-16540
Chemical labeling of intracellular proteins via affinity conjugation and strain-promoted cycloaddition.
Link to the article

D. Schumacher, J. Helma, F.A. Mann, G. Pichler, F. Natale, E. Krause, M.C. Cardoso, C.P.R. Hackenberger, H. Leonhardt
Angew. Chem. Int. Ed.
2015, 54(46), 13787-13791
Versatile and efficient site-specific protein functionalization by tubulin tyrosine ligase
Link to the article

M. Rajan, O. Mortusewicz, U. Rothbauer, F.D. Hastert, K. Schmidthals, A. Rapp, H. Leonhardt
FEBS Open Bio.
2015, 5, 779 - 788
Generation of an alpaca-derived nanobody recognizing y-H2AX.
Link to the article

K. Klamecka, P.M. Severin, L.F. Milles, H.E. Glaub, H. Leonhardt
Phys. Biol.
2015, 12, 056009
Energy profile of nanobody-GFP complex under force.
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U. Reinhardt, J. Lotze, K. Mörl, A.G. Beck-Sickinger, O. Seitz
Bioconjug. Chem.
2015, 26, 2106-2117
Rapid Covalent Fluorescence Labeling of Membrane Proteins on Live Cells via Coiled-Coil Templated Acyl Transfer.
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J.E. Hoffmann, T. Plass, I. Nikic, I.V. Aramburu, C. Köhler, H. Gillandt, E.A. Lemke, C. Schultz
2015, 21(35), 12266-12270
Highly stable trans-cyclooctene amino acids for live cell labeling
Link to the article

P.O. Strale, L. Duchesne, G. Peyret, L. Montel, T. Nguyen, E. PNG, R. Tampé, S. Troyanovsky, S. Hénon
J Cell Biol
2015, 210, 333-346
The formation of ordered nano-clusters controls cadherin anchoring to actin and cell-cell contact fluidity.
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O. Jost, A. Hanswillemenke, D. Schwarzer
Mol Biosyst.
2015, 11, 1820-1823
A miniaturized readout strategy for endogenous histone ceacetylase activity.
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R.B. Quast, D. Mrusek, C. Hoffmeister, A. Sonnabend, S. Kubick
FEBS letters
2015, 589 (15), 1703-1723
Cotranslational incorporation of non-standard amino acids using
cell-free protein synthesis
Link to the article

S. Dickgiesser, N. Rasche, D. Nasu, S. Middel, S. Hörner, O. Avrutina, U. Diederichsen, H. Kolmar
ACS Chem-Biol.
2015, 10(9), 2158-2165
Self-Assembled Hybrid Aptamer-Fc Conjugates for Targeted Delivery: A Modular Chemoenzymatic Approach
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J. Helma, M.C. Cardoso, S. Muyldermans, H. Leonhardt
J. Cell. Biol.
2015, 209, 633 - 644.
Nanobodies and recombinant binders in cell biology.
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S. Hörner, C. Uth, O. Avrutina, H. Frauendorf, M. Wiessler, H. Kolmar
Chem. Commun.
2015, 51, 11130
Combination of inverse electron-demand Diels-Alder reaction with highly efficient oxime ligation expands the toolbox of site-selective peptide conjugations
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K. Heller, P. Ochtrop, M.F. Albers, F.B. Zauner, A. Itzen, C. Hedberg
Angew. Chem. Int. Ed.
2015, 54, 10327-10330
Covalent protein labeling by enzymatic phosphocholination
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D. Wiegandt, S. Vieweg, F. Hofmann, D. Koch, F. Li, Y. Wu, A. Itzen, M.P. Müller, R.S. Goody
Nature Communications 2015, 6, 7773
Locking GTPases covalently in their functional states
Link to the article

R. Wieneke, A. Raulf, A. Kollmannsperger, M. Heilemann, R. Tampé
Angew. Chem. Int. Ed.
2015, 54, 10216-10219
SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging

S. Tyagi, E.A. Lemke
Curr. Opin. Struct. Biol.
2015, in press
Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids.
Link to the article

M. Mühlberg, M.G. Hösl, C. Kühne, J. Dernedde, N. Budisa, C.P.R. Hackenberger
Beilstein JOC
2015, 11, 784-791
Orthogonal Dual-Modification of Proteins for the Engineering of Multivalent Protein Scaffolds
Link to the article

M. Best, A. Degen, T.T. Schmidt, M. Baalmann, R. Wombacher
2015, 16, 1158-1162.
Two-Step Protein Labeling by Using Lipoic Acid Ligase with Norbornene Substrates and Subsequent Inverse-Electron Demand Diels-Alder Reaction.
Link to the article

C. Nadler, A. Nadler, C. Hansen, U. Diederichsen
Eur. J. Org. Chem.
2015, 14, 3095-3102.
A novel photocleavable auxiliary for Extended Native Chemical Ligation

F. Maass, J. Wüstehube-Lausch, S. Dickgießer, B. Valldorf, M. reinwarth, H.-U. Schmoldt, M. Daneschdar, O. Avrutina, U. Sahin, H. Kolmar
J. Pept. Sci.
2015, 8, 651-660
Cystine-knot peptides targeting cancer-relevant human cytotoxic T lymphocyte-associated antigen 4 (CTLA-4)
Link to the article

G. Kubik, D. Summerer
ACS Chem. Biol.,
2015, accepted
Deciphering epigenetic cytosine modifications by direct molecular recognition
Link to the article

K. Rohmer, J. Mannuthodikayil, V. Wittmann
Isr. J. Chem.
2015, 55( 3-4), 437-446
Application of the Thioacid-Azide Ligation (TAL) for the Preparation of Glycosylated and Fluorescently Labeled Amino Acids
Link to the article

A. Schreiber, M.C. Huber, H. Cölfen, S.M. Schiller
Nature Comm.
2015, DOI: 10.1038/ncomms7705
Molecular protein adaptor with genetically encoded interaction-sites guiding the hierarchical assembly of plasmonically active nanoarchitectures
Link to the article

R.B. Quast, O. Kortt, J. Henkel, S.K. Dondapati, D.A. Wüstenhagen, M. Stech, S. Kubick
J. of Biotech.
2015, 203, 45-53
Automated production of functional membrane proteins using eukaryotic cell-free translation systems
Link to the article

L. Schmohl, F.R. Wagner, M. Schümann, E. Krause, D. Schwarzer
Bioorg. Med. Chem.
2015, 15, 2883-2889
Semisynthesis and initial characterization of sortase A mutants containing selenocysteine and homocysteine.
Link to the article

C. Witte, V. Martos, H.M. Rose, R. Reinke, S. Klippel, L. Schröder, C.P.R. Hackenberger
Angew. Chem. Int. Ed.
2015, 54(9), 2806-2810 (VIP-Paper)
Live-cell MRI of xenon Hyper-CEST biosensors targeted in metabolically-labeled cell-surface glycans
Link to the article

N. Nischan, H.D. Herce, F. Natale, N. Bohlke, N. Budisa, M.C. Cardoso, C.P.R. Hackenberger
Angew. Chem. Int. Ed.
2015, 54(6), 1950-1953
Covalent Attachment of Cyclic TAT Peptides to GFP Results in Protein Delivery into Live Cells with Immediate Bioavailability
Link to the article

K.M. Schelkle, T. Griesbaum, D. Ollech, S. Becht, T. Buckup, M. Hamburger, R. Wombacher
Angew. Chem. Int. Ed. 2015, 54(9), 2825-2829
Light-Induced Protein Dimerization by One- and Two-Photon Activation of Gibberellic Acid.
Link to the article

R. Meyer, A. Faesen, K. Vogel, S. Jeganathan, A. Musacchio, C.M. Niemeyer
Small 2015, in press
DNA-Directed Assembly of Capture Tools for Constitutional Studies of Large Protein Complexes.
Link to the article

M.C. Huber, A. Schreiber, P. von Olshausen, B.R. Varga, O. Kretz, B. Joch, S. Barnert, R. Schubert, S. Eimer, P. Kele, S.M. Schiller
Nature Chemicals
2015, 14(1), 125,132
Designer amphiphilic proteins as building blocks for the intracellular formation of organelle-like compartments
Link to the article

O. Reimann, C. Smet-Nocca, C.P.R. Hackenberger
. Chem. Int. Ed. 2015, 54, 306-310
Traceless Purification and Desulfurization of Tau Protein Ligation Products
Link to the article

M.R.J. Vallée, P. Majkut, D. Krause, M. Gerrits, C.P.R. Hackenberger
Chem. Eur. J.
2015, 21, 970-974
Chemoselective Bioconjugation of Triazole Phosphonites in Aqueous Media
Link to the article

A.-L. Bachmann, J.C. Matern, V. Schütz, H.D. Mootz
Methods Mol. Biol.
2015, 1266, 145-159
Chemical-tag labeling of proteins using fully recombinant split inteins
Link to the article

J.C. Matern, A.-L. Bachmann, I.V. Thiel, G. Volkmann, A. Wasmuth, J. Binschik, H.D. Mootz
Methods Mol. Biol.
2015, 1266, 129-143
Ligation of Synthetic Peptides to Proteins Using Semisynthetic Protein trans-Splicing
Link to the article

C. Hedberg, A. Itzen
ACS Chem. Biol.
2015, 10, 12-21
Molecular perspectives on protein adenylylation.
Link to the article

M. Stech, S. Kubick
2015, 4, 12-33
Cell-Free Synthesis Meets Antibody Production: A Review
Link to the article

G. Kubik, D. Summerer
2015, 16(2), 228-231
Achieving single nucleotide resolution of 5-methylcytosine detection with TALEs
Link to the article

G. Kubik, S. Batke, D. Summerer
J. Am. Chem. Soc.
2015, 137, 2-5
Programmable sensors of 5-hydroxymethylcytosine
Link to the article


D. Aschenbrenner, D.A. Pippig, K. Klamecka, K. Limmer, H. Leonhardt, H.E. Gaub
PLoS One 2014, e115049
Parallel force assay for protein-protein interactions.
Link to the article

M. Pott, M.J. Schmidt, D. Summerer
ACS Chem. Biol.
2014, 9(12), 2815-2822
Evolved sequence contexts for highly efficient amber suppression with noncanonical amino acids
Link to the article

A. Yang, L. Zhao, Y.W. Wu
Topics Curr. Chem.
2014, 362, 137-182
Chemical synthesis and biological function of lipidated proteins.
Link to the article

A.-K. Späte, V.F. Schart, S. Schöllkopf, A. Niederwieser, V. Wittmann
Chem. Eur. J.
2014, 20 (50), 16502-16508
Terminal Alkenes as Versatile Chemical Reporter Groups for Metabolic Oligosaccharide Engineering
Link to the article

H.D. Herce, A.E. Garcia, M.C. Cardoso
J. Am. Chem. Soc.
2014, 136, 17459-17467
Fundamental molecular mechanism for the cellular uptake of guanidinium-rich molecules
Link to the article

A. Reif, S. Siebenhaar, A. Tröster, M. Schmälzlein, C. Lechner, P. Velisetty, K. Gottwald, C. Pöhner, I. Boos, V. Schubert, S. Rose-John, C. Unverzagt
Angew. Chem.
2014, 126, 12321-12327
Angew. Chem. Int. Ed. 2014, 53, 12125-12131
Semisynthesis of Biologically Active Glycoforms of the Human Cytokine Interleukin 6
Link to the article

M. Huber, A. Schreiber, K. Benz, S.M. Schiller
2014, 35,31, 8767-8779
Introducing a combinatorial DNA-toolbox platform constituting defined protein-based biohybrid materials
Link to the article

L. Schmohl, D. Schwarzer
Curr. Opin. Chem. Biol.
2014, 22, 122-128
Sortase-mediated ligations for the site-specific modification of proteins
Link to the article

D. Schumacher, C.P.R. Hackenberger
Curr. Opin. Chem. Biol.
2014, 22, 62-69
More than add-on: chemoselective reactions for the synthesis of functional peptides and proteins
Link to the article

R. Wieneke, N. Labòria, M. Rajan, A. Kollmannsperger, F. Natale, M.C. Cardoso, R. Tampé
J. Am. Chem. Soc.
2014, 136, 13975-13978
Live-cell Targeting of His-tagged Proteins by Multivalent N-Nitrilotriacetic Acid Carrier Complexe
Link to the article

P. Liu, A. Calderon, J. Hou, G. Konstantinidis, X. Chen, L. Fu, S. Banerjee, I. Dehmelt, Y.W. Wu
Angew. Chem. Int. Ed.
2014, 53(38), 10049-10055
A bioorthogonal small-molecule switch system for controlling protein function in cells
Link to the article

V. te Kamp, H.-G. Jahnke, D. Krinke, K.B. Kostelnik, A.G. Beck-Sickinger, A.A. Roblitzki
Biosens Bioelectron
2014, 14, 662-669
Quantitative impedimetric NPY-receptor activation monitoring and signaling profiling in living cells.
Link to the article

J. Bertran-Vicente, R.A. Serwa, M. Schümann, P. Schmieder, E. Krause, C.P.R. Hackenberger
J. Am Chem. Soc.
2014, 136(39), 13622-13628
Site-Specifically Phosphorylated Lysine Peptides
Link to the article

E.A. Lemke
2014, 15(12), 1691-1694
The exploding genetic code.
Link to the article

V. Mäde, K. Bellmann-Sickert, A. Kaiser, J. Meiler, A.G. Beck-Sickinger
2014, 9, 2463-2474
Position and length of fatty acids strongly affect receptor selectivity pattern of human pancreatic polypeptide analogues.
Link to the article

M.J. Schmidt, A. Weber, M. Pott, W. Welte, D. Summerer
, 2014, in press
Structural basis of furan-amino acid recognition by a polyspecific aminoacyl-tRNA-synthetase for its genetic encoding in human cells
Link to the article

S. Hauke, M. Best, T.T. Schmidt, M. Baalmann. A. Krause, R. Wombacher
, 2014, 25(9), 1632-1637.
Two-Step Protein labeling Utilizing Lipoic Acid Ligase and Sonogashira Cross-Coupling
Link to the article

U. Reinhardt, J. Lotze, S. Zernia, K. Mörl, A.G. Beck-Sickinger, O. Seitz
Angew. Chem. Int. Ed.
2014, 53, 10237-10241.
Peptide-templated acyl transfer: a chemical method for the labeling of membrane proteins.
Link to the article

V. Mäde, S. Babilon, N. Jolly, L. Wanka, K. Bellmann-Sickert, L.E. Diaz Gimenez, K. Mörl, H.M. Cox, V.V. Gurevich, A.G. Beck-Sickinger
Angew. Chem. Int. Ed.
2014, 53, 10067-10071
Peptide modifications differentially alter G protein-coupled receptor internalization and signaling bias.
Link to the article

Y. Wang, D. Rösner, M. Grzywa, A. Marx
Angew. Chem. Int. Ed. 2014, 53, 8159-8162.
Chain terminating and clickable NAD+ analogues for labeling target proteins of ADP-ribosyltransferases
Link to the article

K.A. Gelato, M. Tauber, M.S. Ong, S. Winter. K. Hiragami-Hamada, J. Sindlinger, A. Lemak, Y. Bultsma, S. Houliston, D. Schwarzer, N. Divecha, C.H. Arrowsmith, W. Fischle
Mol. Cell
2014, 54, 905-919
Accessibility of Different Histone H3-Binding Domains of UHRF1 Is Allosterically Regulated by Phosphatidylinositol 5-Phosphate.
Link to the article

G. Kubik, M.J. Schmidt, J.E. Penner, D. Summerer
Angew. Chem. Int. Ed.
, 2014, 53(23), 6002-6006.
Programmable and highly resolved detection of 5-methylcytosine by TALE-controlled DNA replication
Link to the article

H. Fittler, O. Avrutina, M. Empting, H. Kolmar
J Pept sci. 2014, 20(6), 415-420
Potent inhibitors of human matriptase-I based on the scaffold of sunflower trypsin inhibitor.
Link to the article

S. Liebscher, P. Kornberger, G. Fink, E.-M. Trost-Gross, E. Höss, A. Skerra, F. Bordusa
2014, 15, 1096-1100
Derivatization of Antibody Fab Fragments: A Designer Enzyme for Native Protein Modification
Link to the article

V. Gatterdam, R. Ramadass, T. Stoess, M.A.H. Fichte, J. Wachtveitl, A. Heckel, R. Tampé
Angew. Chem. Int. Ed.
2014, 53(22) 5680-5684
Three-dimensional protein networks guided by two-photon activation
Link to the article

S.F. Frenz, R. Sachse, T. Schmidt, S. Kubick
Biochimica et Biophysica Acta
2014, 1838, 1382-1388
Cell-free synthesis of membrane proteins: Tailored cell models out of microsomes
Link to the article

C. Pöhner, v. Ullmann, R. Hilpert, E. Samain, C. Unverzagt
Tetrahedron Lett.
2014, 55, 2197-2200
Chemoselective coupling of sugar oximes and α-ketoacids to glycosyl amides and N-glycopeptides
Link to the article

A. Wieczorek, T. Buckup, R. Wombacher
2014, 12 (24), 4177-4185
Rigid tetrazine fluorophore conjugates with fluorogenic properties in inverse electron demand Diels-Alder reaction
Link to the article

V. Schütz, H.D. Mootz
Angew. Chem. Int. Ed.
2014, 126 (16), 4197-4201
Click-tag and amine-tag: New chemical tag approaches for efficient protein labeling in vitro and on live cells using the naturally split Npu DnaE intein
Link to the article

R.B. Quast, I. Clausnitzer, H. Merk, S. Kubick, M. Gerrits
Analytical Biochemistry
2014, 451, 4-9
Synthesis and site-directed fluorescence labeling of azido proteins using eukaryotic cell-free orthogonal translation systems
Link zum Artikel

S. Klopries, K. Koopmans, E. Sanchez-Garcia, F. Schulz
ChemBioChem 2014, 15(4), 495-497
Biosynthesis with Flourine
Link to the article

W. Liu, F. Li, X. Chen, L. Yi, Y.W. Wu
J. Am Chem Soc 2014, 136(12), 4468-4471
A rapid and fluorogenic TMP-AcBOPDIPY probe for covalent labeling of proteins in live cells
Link zum Artikel

S.K. Dondapati, M. Kreir, R.B. Quast, D.A. Wüstenhagen, A. Brüggemann, N. Fertig, S. Kubick
Biosens Bioelectron
2014, 59, 174-183
Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system.
Link zum Artikel

S. Liebscher, M. Schöpfel, T. Aumüller, A. Sharkhuukhen, A. Pech, E. Höss, C. Parthier, G. Jahreis, M.T. Stubbs, F. Bordusa
Angew. Chem. Int. Ed.
2014, 53, 3024-3028
N-Terminal Protein Modification by Substrate-Activated Reverse Proteolysis
Link to the article

I. Nikic, T. Plass, O. Schraidt, J. Szymanski, J.A. Briggs, C. Schultz, E.A. Lemke
Angew. Chem. Int. Ed.
2014, 53 (8), 2245-2249
Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy
Link zum Artikel

M.J. Schmidt, D. Summerer
Front. Chem.
2014, 2(7), 1-11
Genetic code expansion as a tool to study regualatory processes of trascription
Link zum Artikel

L. Schmohl, D. Schwarzer
J. Pept. Sci.
2014, 20, 145-151
Chemo-enzymatic three-fragment assembly of semisynthetic proteins.
Link to the article

F. Li, I. Yi, I Zhao, A. Itzen, R.S. Goody, Y.W. Wu
Proc. Natl. Acad. Sci. U.S.A.
2014, 111(7), 2572-2577
The role of the hypervarialbe C-terminal domain in Rab membrane targeting
Link zum Artikel

I.V. Thiel, G. Volkmann, S. Pietrokovski, H.D. Mootz
Angew. Chem. Int. Ed.
2014, 53, 1306-1310
An atypical naturally split intein engineered for highly efficient protein labeling
Link zum Artikel

S. Voss, L. Zhao, X. Chen, F. Gerhard, Y.W. Wu
J. Pep. Sci. 2014, in press
Generation of an intramolecular three-color FRET probe by site-specific protein labeling
Link zum Artikel

M.P. Müller, M.F. Albers, A. Itzen, C. Hedberg
2014, 15(1), 19-26
Exploring Adenylylation and Phosphocholinaton as Post-Translational Modifications
Link zum Artikel

M.J. Schmidt, J. Borbas, M. Drescher, D. Summerer
J. Am. Chem. Soc.
2014, 136, 1238-1241
A genetically encoded spin label for electron paramagnetic resonance distance measurements
Link zum Artikel

A.-K. Späte, H. Bußkamp, A. Niederwieser, V. F. Schart, A. Marx, V. Wittmann
Bioconjug Chem.
2014, 25, 147-154
Rapid Labeling of Metabolically Engineered Cell-Surface Glycoconjugates with a Carbamate-Linked Cyclopropene Reporter
Link to the article


P. Majkut, I. Claußnitzer, H. Merk, C. Freund, C.P.R. Hackenberger, M. Gerrits
2013, 8(12): e82352
Completion of Proteomic Data Sets by Kd Measurement Using Cell-Free Synthesis of Site-Specifically Labeled Proteins
Link zum Artikel

H.D. Herce, W. Deng, J. Helma, H. Leonhardt, M.C. Cardoso
Nat. Commun 2013, 4, 2660
Visualization and targeted disruption of protein interactions in living cells.
Link to the article

B. Glotzbach, M. Reinwarth, N. Weber, S. Fabritz, M. Tomaszowski, H. Fittler, A. Christmann, O. Avrutina, H. Kolmar
PLoS One 2013, 8(10):e76956. DOI: 10.1371/journal.pone.0076956
Combinatorial optimization of cystine-knot peptides towards high-affinity inhibitors of human matriptase-1.

Link zum Artikel

S. Bechlars, D.A. Wüstenhagen, K. Drägert, R. Dieckmann, E. Strauch, S. Kubick
Toxicon 2013, 76, 132-142
Cell-free synthesis of functional thermostable direct hemolysins of Vibrio parahaemolyticus
Link zum Artikel

N. Nischan, A. Chakrabarti, R.A. Serwa, P.H.M. Bovee-Geurts, R. Brock, C.P.R. Hackenberger
Angew. Chem.
2013, 125, 45, 12138-12142
Stabilisierung von Peptiden für intrazelluläre Anwendungen mit Phosphoramidat-verzweigten Polyethylenglycol-Ketten
Angew. Chem. Int. Ed. 2013, 52, 45, 11920-11924
Stabilization of Peptides for Intracellular Applications by Phosphoramidate-Linked PEG Chains
Link zum Artikel

A. Yang, Y. Li, S. Pantoom, G. Triola, Y.W. Wu
2013, 14(11), 1296-1300
Semisynthetic Lipidated LC3 Protein Mediates Membrane Fusion
Link zum Artikel

M.R.J. Vallée, L.M. Artner, J. Dernedde, C.P.R. Hackenberger
Angew. Chem.
2013, 125, 9682-9686
Angew. Chem. Int. Ed.
2013, 52, 9504-9508
Alkyne phophonites for sequential azide-azide couplings
Link zum Artikel

C. Unverzagt, Y. Kajihara
Chem. Soc. Rev
. 2013, 42, 4408-4420
Chemical assembly of N-glycoproteins: a refined toolbox to address a ubiquitous posttranslational modification
Link zum Artikel

A. Herner, I. Nikic, M. Kallay, E.A. Lemke, P. Kele
Org Biomol Chem
2013, 11(20), 3297-3306
A new family of bioorthogonally applicable fluorogenic labels.
Link zum Artikel

M.J. Schmidt, D. Summerer
Angew. Chem. Int. Ed.
2013, 52, 4690-4693
Red-light-controlled protein-RNA crosslinking with a genetically encoded furan
Link zum Artikel

M.F. Albers, C. Hedberg
J. Org. Chem.
2013, 78(6), 2715-2719
Amino Acid Building Blocks for Fmoc Solid-Phase Synthesis of Peptides Phosphocholinated at Serine, Threonine, and Tyrosine
Link zum Artikel

N. Labòria, R. Wieneke, R. Tampé
Angew. Chem. Int. Ed. 2013, 52, 848-853
Control of Nanomolar Interaction and In Situ Assembly of Proteins in Four Dimensions by Light

B. Glotzbach, S. Schmelz, M. Reinwarth, A. Christmann, D.W. Heinz, H. Kolmar
Acta Crystallogr D Biol Crystalogr 2013, 69(Pt 1):114-20. DOI: 10.1107/S0907444912043880
Structural characterization of Spinacia oleracea trypsin inhibitor III (SOTI-III)

Link zum Artikel

S. Milles, E.A. Lemke
Bioessays 2013, 35(1), 65-74
What precision-protein-tuning and nano-resolved single molecule sciences can do for each other
Link zum Artikel


V. Ullmann, M. Rädisch, I. Boos, J. Freund, C. Pöhner, S. Schwarzinger, C. Unverzagt
Angew. Chem. Int. Ed.
2012, 51, 11566-11570
Convergent Solid Phase Synthesis of N-Glycopeptides supported by Pseudoprolines at Consensus Ser/Thr Residues
Link zum Artikel

D. Ott, J. Seifert, I. Prahl, M. Niemietz, J. Hoffman, J. Guder, M. Mönnich, C. Unverzagt
Eur. J. Org. Chem.
2012, 26, 5054-5068
Modular Synthesis of Core Fucosylated N-Glycans
Link zum Artikel

M. Stech, H. Merk, J.A. Schenk, W.F.M. Stöcklein, D.A. Wüstenhagen, B. Micheel, C. Duschl, F.F. Bier, S. Kubick
J. of Biotechnology
2012, 164, 220-231
Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system
Link zum Artikel

D.E. Zampatis, C. Rutz, J. Furkert, A. Schmidt, D. Wüstenhagen, S. Kubick, N.E. Tsopanoglou, R. Schülein
FEBS Letters 2012, 586, 2351-2359
The protease-activated receptor 1 possesses a functional and cleavable signal peptide which is necessary for receptor expression
Link zum Artikel