Tampé

Cellular Logistics - 'Traceless' protein modification by small high-affinity tags in living cells

Labeling and modification of intracellular proteins remain a major challenge in life sciences, often limited by large fusion domains, high quantities of costly probes, and long reaction times. Here, we will guide protein trans-splicing by minimal high-affinity interaction pairs to trace proteins´ function and assembly in living cells. In addition, we aim at modulating cellular processes by light with high resolution in time and space. Combined with super-resolution microscopy and single-molecule analysis, these traceless approaches shall provide valuable insights into the co-operative function and suprastructures of proteins in particular at intracellular membranes.

Prof. Dr. Robert Tampé
Goethe-Universität Frankfurt am Main

Tel.: +49 69 798 29475
Fax: +49 69 798 29495

Email Prof. Tampé

Publications within the SPP 1623 project

A. Blees, D. Januliene, T. Hofmann, N. Koller, C. Schmidt, S. Trowitzsch, A. Moeller, R. Tampé
Nature
2017, 551, 525-528
Structure of the human MHC-I peptide-loading complex
Link to the article

M. Braner, R. Wieneke, R. Tampé
Chem.Commun.
2017, 53, 545-548
Nanomolar affinity protein trans-splicing monitored in real-time by fluorophore-quencher pairs.
Link to the article

A. Guesdon, F. Bazile, R.M. Buey, R. Mohan, S. Monier, R.R. Garcia, M. Angevin, C. Heichette, R. Wieneke, R. Tampé, L. Duchesne, A. Akhmanova, M.O. Steinmetz, D. Chrétien
Nature Cell Biology
2016, 8, 1102-1108
EB1 interacts with outwardly curved and straight regions of the microtubule lattice.
Link to the article

A. Kollmannsperger, A. Sharei, A. Raulf, M. Heilemann, R. Langer, K.F. Jensen, R. Wieneke, R. Tampé
Nature Commun.
2015, 7, 10372
Live-cell protein labelling with nanometre precision by cell squeezing
Link to the article

M. Braner, A. Kollmannsperger, R. Wieneke, R. Tampé
Chem.Sci.
2015, 7, 2646-2652
'Traceless' tracing of proteins - high-affinity transsplicing directed by a minimal interaction pair.
Link to the article

M. Pfreundschuh,D. Alsteens, R. Wieneke, C. Zhang, S. Soughlin, R. Tampé, B. Kobilka, D. Müller
Nature Commun
2015, 6, 8857
Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM.
Link to the article

R. Wieneke, A. Raulf, A. Kollmannsperger, M. Heilemann, R. Tampé
Angew. Chem. Int. Ed.
2015, 54, 10216-10219
SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging
Link to the article

P.O. Strale, L. Duchesne, G. Peyret, L. Montel, T. Nguyen, E. PNG, R. Tampé, S. Troyanovsky, S. Hénon
J Cell Biol
2015, 210, 333-346
The formation of ordered nano-clusters controls cadherin anchoring to actin and cell-cell contact fluidity.
Link to the article

R. Wieneke, N. Labňria, M. Rajan, A. Kollmannsperger, F. Natale, M.C. Cardoso, R. Tampé
J. Am. Chem. Soc.
2014, 136, 13975-13978
Live-cell Targeting of His-tagged Proteins by Multivalent N-Nitrilotriacetic Acid Carrier Complexe
Link to the article

V. Gatterdam, R. Ramadass, T. Stoess, M.A.H. Fichte, J. Wachtveitl, A. Heckel, R. Tampé
Angew. Chem. Int. Ed.
2014, 53(22) 5680-5684
Three-dimensional protein networks guided by two-photon activation
Link to the article

N. Labňria, R. Wieneke, R. Tampé
Angew. Chem. Int. Ed. 2013, 52, 848-853
Control of Nanomolar Interaction and In Situ Assembly of Proteins in Four Dimensions by Light