1.1. Strategies for the synthesis of functional proteins

The development of chemoselective ligation- and modification strategies plays an important role in the current research of protein synthesis. These strategies can be based on chemical and enzymatic methods but also include the biochemical synthesis of protein fragments.3 A clever combination of chemical and biochemical processes and tools is the only way to achieve selective placement of modified (e.g. glycosylated or phosphorylated) amino acids or attach biophysical probes within a complex biomolecule.5

Scheme 1 wants to give an overview of the methodical basis for the chemoselective synthesis of proteins. Moreover, four important subfields and tools are shown that can be combined and used for the synthesis of very complex biomolecules. These four tools will be further discussed on the following pages (see 1.1.1-4).

Scheme 1